Read e-book online Animal Cell Biotechnology: Methods and Protocols PDF

By Nigel Jenkins

ISBN-10: 0896035476

ISBN-13: 9780896035478

Eli Lilly & Co., Indianapolis, IN. stories fresh examine and covers new strategies utilized in genetically manipulating animal cells, optimizing their development in outlined media, warding off infection, and in harvesting and reading of cellphone items. For researchers.

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Robey, W G , Oroszlan, S D , and Tsar, W P. (1985) Isolatton from cats of an endogenous type C virus with a novel envelope glycoprotem J Vwol 53,827-833 27. Bassin, R. H , Tuttle, N , and Fischmger, P. J (1971) Raptd cell culture assay technique for murme leukaemia virus. , Rowe, W. , and Pugh, W. E (1969) Mixed culture cytopathogemctty: a new test for growth of murine leukaemta virus m tissue culture. Proc Natl. Acad. Scl USA 63,753-758 29 Lander, M. R. and Chattopadhyay, S K (1984). &s dunnz cell line that lacks sequences closely related to endogenous murine leukaemta vuuses and can be infected by ecotropic, amphotropic, xenotropic and mmk cell focus-forming viruses.

Plasmid maps for in vitro amplification. Cloning vectors pStXV19 and pCISfiT carry multiple cloning sites downstream of the SV40 early and CMV immediate-early (IE) promoters, respectively. Selectable marker plasmids pSfiSVneo and pStiSVdhfr are both under the SV40 early promoter and carry the neomycin resistance (NEO) and the dihydrofolate reductase (dhfr) genes, respectively. Polyadenylation sites are either from SV40 early (pSfiSV19, pSfiSVneo, pSfiSVdhfr) or SV40 late (pCISfiT) regions. A transcription terminator sequence from the human gastrin gene has been inserted in pCIStiT.

This numFrom Methods m Botechnology, Vol 8 Anmal Cell Bfotechnology E&ted by N Jenkins 0 Humana Press Inc , Totowa, NJ 49 Wurm and Schiffmann 50 ber may fluctuate shghtly durmg the course of subcultlvatlon, a phenomenon, that 1sobserved m all immortalized cell lines In general, CHO cells are considered to have a quite stable karyotype. It must be noted however, that CHO cell populations selected for MTX resistance and subcultivated m the presence of a drug for extended periods often contam substantial subpopulations of cells with increased chromosome numbers.

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Animal Cell Biotechnology: Methods and Protocols by Nigel Jenkins

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